Possible Clues:

• Note essential to Purcell?
• Centrally located note
• Good note for beginners to start on
• First line below the treble stave
• Reference point during a piano lesson
• Perfectly hit Chopin's first note
• First note learned on the piano, and a homophonic hint to the starred answers
• Note what act has?
• Starting point for a piano student, or a phonetic hint to 17-, 18-, 22-, 33-, 41-, 49- and 55-Across
• Note what Tuscany has?