Possible Clues:

• Relatively ugly characters in 1 down
• Is in relatively twisted tress for them
• Meg, Jo, Beth and Amy of 'Little Women,' e.g
• Resists abnormally religious women
• Second and first queen's relatives
• Nuns stress terribly crossing Italy
• Venus and Serena, e.g
• The Pleiades, e.g
• Female associates
• Muses say hr/3600 is briefer than brief