Possible Clues:

• Third U.S. VP
• Hamilton dueler Aaron
• Rotary file — feature of some regional accents
• Lilt, accent
• First vice president not to become president
• Alexander Hamilton foe
• Something rough in Mexican dish, one to cut out
• "Wait For It" singer in "Hamilton"
• Hamilton's foe
• Seedcase donkey's bitten off at the end