Possible Clues:

• Sounds in pain that has got worse, by the sound of it
• Grandmother's eaten nothing - complain
• Aged relative accepted ring - with complaint
• Onset of glanders - horse's painful sigh
• Titus ---, (Mervyn Peake)
• There's nothing in Granny but pain, by the sound of it
• Good horse providing beef
• It often follows a pun
• Response to a weak joke, perhaps
• Grumble from old lady possessing nothing?